AD-FA

Supplemental Data

“Mitochondrial folate one-carbon metabolism moderates Alzheimer’s disease pathology”

Yizhou Yu, Ivana Celardo, Civia Z. Chen, Bryan Wei Zhi Tan, Nuno Santos Leal, Rebeka Popovic, Samantha H. Y. Loh, and L. Miguel Martins University of Cambridge

Figure 1 - Proteomic analysis of Abeta-Arc-expressing flies compared to controls

This folder contains the processed proteomics data and the scripts used for the subsequent analyses.

Supplemental Table 1 - Full list of proteins detected in Abeta-Arc-expressing flies and controls

Figure 3 - High-throughput analysis of sleep and lifespan in Abeta-expressing flies

This folder contains the raw data data from Drosophila Activity Monitors (DAM2) and the scripts for the subsequent analyses.

Figures 4 and 7 - UK Biobank analysis of MTHFD2L SNPs and folate intake in AD patients and controls

This folder contains only the analysis scripts. Please contack the UK Biobank (link) to obtain data access. We are happy to transfer the processed data upon approval from the UK Biobank.

Figure 5 - Single-cell RNA sequencing of neurons from AD and controls, and single-cell mendelian randomisation of MTHFD2L in neurons

This folder contains script for single cell RNA analysis of MTHFD2L expression. The raw data is obtained from the cellxgene platform. All anlaysis scripts are in the folder. This folder also contains the data for the single cell Mendelian randomisation analyses i.e. the eQTLs for MTHFD2L expression, harmonised data and the scripts for plotting.

These are the folders of the analyses

Figure 6 & 8 - High-throughput analysis of sleep and lifespan in Abeta-expressing flies fed with folate

This folder contains the raw data data from Drosophila Activity Monitors (DAM2) and the scripts for the subsequent analyses.